HSPA9 antibody (AA 551-766) (FL490)

Catalog No. ABIN7595463

The Mouse Monoclonal anti-HSPA9 antibody has been validated for IHC and ICC. It is suitable to detect HSPA9 in samples from Mouse.

Quick Overview for HSPA9 antibody (AA 551-766) (FL490) (ABIN7595463)

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPA9 antibody is conjugated to FL490

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: N52A-42

Product Details anti-HSPA9 Antibody (FL490)

Binding Specificity: AA 551-766

Purpose: Anti-Mortalin/GRP75 Antibody FL490 Conjugate

Specificity: No off-targets reported

Cross-Reactivity: Human, Mouse, Non-Human Primate, Rat

Characteristics:

Description: Our Anti-Mortalin/GRP75 mouse monoclonal primary antibody is produced in-house from hybridoma clone N52A/42. It detects human, mouse, and rat Mortalin/GRP75, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Manufacturer Comment: We produce our Mortalin/GRP75 mouse monoclonal primary antibody from hybridoma clone N52A/42. It is great in IHC, ICC and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb.

Purity: > 90 % specific antibody

Immunogen: Identified as off-target mAb in screen for anti-Salm2 NeuroMabs (immunogen was fusion protein amino acids 551-766 of mouse SALM2). Target identified as Mortalin/Grp75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 12 months

Target Details for HSPA9

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Alternative Name: GRP75

Background:

Synonyms: Stress-70 protein, mitochondrial (75 kDa glucose-regulated protein) (GRP-75) (Heat shock 70 kDa protein 9) (Mortalin) (Peptide-binding protein 74) (PBP74) (p66 MOT)

Target Description: Mitochondrial stress-70 protein or Mortalin/GRP75 is encoded by the gene HSPA9. Mortalin is a chaperone protein and plays a role in cell proliferation, stress response and mitochondria maintenance. It is widely expressed in tissues in mitochondria, endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. Diseases associated with this gene include Even-Plus Syndrome and Sideroblastic Anemia 4.

Gene Name Alternatives: Hspa9 Grp75 Hsp74 Hspa9a

Molecular Weight: 75 kDa