DNMT3L antibody (AA 1-387) (FL650)

Catalog No. ABIN7595807

This Mouse Monoclonal antibody specifically detects DNMT3L in ICC. It exhibits reactivity toward Human.

Quick Overview for DNMT3L antibody (AA 1-387) (FL650) (ABIN7595807)

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3L antibody is conjugated to FL650

Application: Immunocytochemistry (ICC)

Clone: N117-9

Product Details anti-DNMT3L Antibody (FL650)

Binding Specificity: AA 1-387

Purpose: Anti-DNMT3L Antibody FL650 Conjugate

Specificity: No off-targets reported for DNMT3A or DNMT3B

Cross-Reactivity: Human

Characteristics:

Description: Our Anti-DNMT3L mouse monoclonal primary antibody is produced in-house from hybridoma clone N117/9. It detects human DNMT3L, and is purified by Protein A chromatography. It is great for use in ICC.

Manufacturer Comment: We produce our DNMT3L mouse monoclonal primary antibody from hybridoma clone N117/9. It is great in ICC and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb.

Purity: > 90 % specific antibody

Immunogen: Fusion protein amino acids 1-387 (full-length) of human DNMT3L (accession number BAA95556) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 12 months

Target Details for DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Alternative Name: DNMT3L

Background:

Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Target Description: DNA (cytosine-5)-methyltransferase 3-like or DNMT3L is encoded by the gene DNMT3L. DNMT3l is a nuclear, catalytically inactive, regulatory factor of DNA methyltransferases. DNMT3L mediates transcriptional repression through interaction with histone deacetylase 1. It is thought to be required for the establishment of maternal genomic imprints. DNMT3L is expressed in several tissues including testis, ovary, and thymus. Diseases associated with DNMT3L include Testicular Spermatocytic Seminoma and Immunodeficiency-Centromeric Instability-Facial Anomalies Syndrome.

Gene Name Alternatives: DNMT3L

Molecular Weight: 45 kDa