eGFP antibody (AA 1-239)

Catalog No. ABIN7596110

The Mouse Monoclonal anti-eGFP antibody (Clone AT1D9) (ABIN7596110) specifically detects eGFP in WB and ELISA. The antibody is reactive with Aequorea victoria samples.

Quick Overview for eGFP antibody (AA 1-239) (ABIN7596110)

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This eGFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: AT1D9

Product Details anti-eGFP Antibody

Binding Specificity: AA 1-239

Purpose: EGFP antibody

Purification: protein-A affinity chromatography

Immunogen: Recombinant EGFP(1-239aa) purified from E. coli

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycero

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details for eGFP

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Alternative Name: EGFP

Background: Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.