PHPT1 antibody (AA 1-125)

Catalog No. ABIN7598972

This anti-PHPT1 antibody is a Rabbit Polyclonal antibody detecting PHPT1 in WB, IHC, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for PHPT1 antibody (AA 1-125) (ABIN7598972)

Target: PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHPT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-PHPT1 Antibody

Binding Specificity: AA 1-125

Purpose: Anti-PHPT1 Antibody Picoband®

Characteristics: Anti-PHPT1 Antibody Picoband® (ABIN7598972). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PHPT1 recombinant protein (Position: M1-Y125). Human PHPT1 shares 84.4% amino acid (aa) sequence identity with mouse PHPT1.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ek, P., Pettersson, G., Ek, B., Gong, F., Li, J.-P., Zetterqvist, O. Identification and characterization of a mammalian 14- kDa phosphohistidine phosphatase. Europ. J. Biochem. 269: 5016-5023, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PHPT1

Target: PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))

Alternative Name: PHPT1

Background: 14 kDa phosphohistidine phosphatase is an enzyme that in humans is encoded by the PHPT1 gene. This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants.

Molecular Weight: 14 kDa

Gene ID: 29085

Pathways: Positive Regulation of Peptide Hormone Secretion