CRYAA antibody (AA 1-173)

Catalog No. ABIN7599031

This Mouse Monoclonal antibody specifically detects CRYAA in WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CRYAA antibody (AA 1-173) (ABIN7599031)

Target: CRYAA (Crystallin, alpha A (CRYAA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 8H6

Product Details anti-CRYAA Antibody

Binding Specificity: AA 1-173

Purpose: Anti-Alpha A Crystallin Antibody Picoband® (monoclonal, 8H6)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Alpha A Crystallin Antibody Picoband® (monoclonal, 8H6) (ABIN7599031). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173). Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse and rat Alpha A Crystallin.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: CRYAA crystallin, alpha A". 2. Derham BK, Harding JJ (1999). "Alpha-crystallin as a molecular chaperone.". Progress in retinal and eye research 18 (4): 463-509. 3. Jaworski CJ, Piatigorsky J (1989). "A pseudo-exon in the functional human alpha A-crystallin gene.". Nature337 (6209): 752-4.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Alternative Name: CRYAA

Background:

Synonyms: Ras-related protein Rab-5A, RAB5A, RAB5

Tissue Specificity: Widely expressed in fetal and adult tissues.

Background: Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed, alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).

Molecular Weight: 20-23 kDa

Gene ID: 1409

UniProt: P02489

Pathways: M Phase