MRPS25 antibody (AA 1-173)

Catalog No. ABIN7599032

The Rabbit Polyclonal anti-MRPS25 antibody has been validated for WB, ELISA, FACS, IHC, IF and ICC. It is suitable to detect MRPS25 in samples from Human, Mouse and Rat.

Quick Overview for MRPS25 antibody (AA 1-173) (ABIN7599032)

Target: MRPS25 (Mitochondrial Ribosomal Protein S25 (MRPS25))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MRPS25 Antibody

Binding Specificity: AA 1-173

Purpose: Anti-MRPS25 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MRPS25 Antibody Picoband® (ABIN7599032). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MRPS25 recombinant protein (Position: M1-D173).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bugiardini, E., Mitchell, A. L., Dalla Rosa, I., Horning-Do, H.-T., Pitmann, A. M., Poole, O. V., Holton, J. L., Shah, S., Woodward, C., Hargreaves, I., Quinlivan, R., Amunts, A., Wiesner, R. J., Houlden, H., Holt, I. J., Hanna, M. G., Pitceathly, R. D. S., Spinazzola, A. MRPS25 mutations impair mitochondrial translation and cause encephalomyopathy. Hum. Molec. Genet. 28: 2711-2719, 2019. 2. Kenmochi, N., Suzuki, T., Uechi, T., Magoori, M., Kuniba, M., Higa, S., Watanabe, K., Tanaka, T. The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders. Genomics 77: 65-70, 2001. 3. Koc, E. C., Burkhart, W., Blackburn, K., Moseley, A., Spremulli, L. L. The small subunit of the mammalian mitochondrial ribosome: identification of the full complement of ribosomal proteins present. J. Biol. Chem. 276: 19363-19374, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MRPS25

Target: MRPS25 (Mitochondrial Ribosomal Protein S25 (MRPS25))

Alternative Name: MRPS25

Background:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: 28S ribosomal protein S25, mitochondrial is a protein that in humans is encoded by the MRPS25 gene. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.

Molecular Weight: 20 kDa

Gene ID: 64432

UniProt: P82663

Pathways: SARS-CoV-2 Protein Interactome