MED28 antibody (AA 1-178)

Catalog No. ABIN7599039

This anti-MED28 antibody is a Rabbit Polyclonal antibody detecting MED28 in ELISA, WB, FACS and IP. Suitable for Human.

Quick Overview for MED28 antibody (AA 1-178) (ABIN7599039)

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED28 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-MED28 Antibody

Binding Specificity: AA 1-178

Purpose: Anti-MED28 Antibody Picoband®

Characteristics: Anti-MED28 Antibody Picoband® (ABIN7599039). Tested in ELISA, Flow Cytometry, IP, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MED28 recombinant protein (Position: M1-T178). Human MED28 shares 94.4% and 94.9% amino acid (aa) sequence identity with mouse and rat MED28, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brass, A. L., Dykxhoorn, D. M., Benita, Y., Yan, N., Engelman, A., Xavier, R. J., Lieberman, J., Elledge, S. J. Identification of host proteins required for HIV infection through a functional genomic screen. Science 319: 921-926, 2008. 2. Liu, C., Zhang, L., Shao, Z.-M., Beatty, P., Sartippour, M., Lane, T. F., Barsky, S. H., Livingston, E., Nguyen, M. Identification of a novel endothelial-derived gene EG-1. Biochem. Biophys. Res. Commun. 290: 602-612, 2002. 3. Lu, M., Zhang, L., Maul, R. S., Sartippour, M. R., Norris, A., Whitelegge, J., Rao, J. Y., Brooks, M. N. The novel gene EG-1 stimulates cellular proliferation. Cancer Res. 65: 6159-6166, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MED28

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Alternative Name: MED28

Background: Mediator of RNA polymerase II transcription subunit 28 is an enzyme that in humans is encoded by the MED28 gene. MED28 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNApolymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatoryproteins to the basal RNA polymerase.

Molecular Weight: 24 kDa

Gene ID: 80306

UniProt: Q9H204

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation