ALAS2 antibody (AA 1-190)

Catalog No. ABIN7599056

The Rabbit Polyclonal anti-ALAS2 antibody is suitable to detect ALAS2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, ICC and FACS.

Quick Overview for ALAS2 antibody (AA 1-190) (ABIN7599056)

Target: ALAS2 (Aminolevulinate, delta-, Synthase 2 (ALAS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ALAS2 Antibody

Binding Specificity: AA 1-190

Purpose: Anti-ALAS2/ASB Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ALAS2/ASB Antibody (ABIN7599056). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALAS2/ASB recombinant protein (Position: M1-D190).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ALAS2

Target: ALAS2 (Aminolevulinate, delta-, Synthase 2 (ALAS2))

Alternative Name: ALAS2

Background:

Synonyms: Endothelin-converting enzyme 1,ECE-1,3.4.24.71,ECE1,

Tissue Specificity: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma, isoforms A and C in liver, testis and small intestine, isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells, isoforms C and D in saphenous vein cells, and isoform C in kidney. .

Background: Delta-aminolevulinate synthase 2 also known as ALAS2 is a protein that in humans is encoded by the ALAS2 gene. The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 65 kDa

Gene ID: 212

UniProt: P22557

Pathways: Transition Metal Ion Homeostasis