APC1 antibody (AA 1-197)

Catalog No. ABIN7599069

This anti-APC1 antibody is a Rabbit Polyclonal antibody detecting APC1 in ELISA, IHC, WB, IF, ICC and FACS. Suitable for Human.

Quick Overview for APC1 antibody (AA 1-197) (ABIN7599069)

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-APC1 Antibody

Binding Specificity: AA 1-197

Purpose: Anti-APC1/ANAPC1 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-APC1/ANAPC1 Antibody Picoband® (ABIN7599069). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human APC1/ANAPC1 recombinant protein (Position: M1-E197). Human ANAPC1 shares 91.9% amino acid (aa) sequence identity with mouse ANAPC1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ajeawung, N. F., Nguyen, T. T. M., Lu, L., Kucharski, T. J., Rousseau, J., Molidperee, S., Atienza, J., Gamache, I., Jin, W., Plon, S. E., Lee, B. H., Teodoro, J. G., Wang, L. L., Campeau, P. M. Mutations in ANAPC1, encoding a scaffold subunit of the anaphase-promoting complex, cause Rothmund-Thomson syndrome type 1. Am. J. Hum. Genet. 105: 625-630, 2019. 2. Jorgensen, P. M., Graslund, S., Betz, R., Stahl, S., Larsson, C., Hoog, C. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene 262: 51-59, 2001. 3. Yang, Y., Kim, A. H., Yamada, T., Wu, B., Bilimoria, P. M., Ikeuchi, Y., de la Iglesia, N., Shen, J., Bonni, A. A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation. Science 326: 575-578, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for APC1

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Alternative Name: ANAPC1

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Anaphase-promoting complex subunit 1 is an enzyme that in humans is encoded by the ANAPC1 gene. This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation.

Molecular Weight: 240 kDa

Gene ID: 64682

UniProt: Q9H1A4