THEM4 antibody (AA 1-240)

Catalog No. ABIN7599140

Product Details anti-THEM4 Antibody

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Binding Specificity: AA 1-240

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THEM4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Anti-THEM4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-THEM4 Antibody Picoband® (ABIN7599140). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human THEM4 recombinant protein (Position: M1-T240).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Maira, S.-M., Galetic, I., Brazil, D. P., Kaech, S., Ingley, E., Thelen, M., Hemmings, B. A. Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane. Science 294: 374-380, 2001. 2. Zhuravleva, E., Gut, H., Hynx, D., Marcellin, D., Bleck, C. K. E., Genoud, C., Cron, P., Keusch, J. J., Dummler, B., Esposti, M. D., Hemmings, B. A. Acyl coenzyme A thioesterase Them5/Acot15 is involved in cardiolipin remodeling and fatty liver development. Molec. Cell. Biol. 32: 2685-2697, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for THEM4

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Alternative Name: THEM4 (THEM4 Products)

Background:

Synonyms: Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27

Tissue Specificity: Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Background: Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase. Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated in the C-terminal regulatory domain. The protein encoded by this gene negatively regulates PKB activity by inhibiting phosphorylation. Transcription of this gene is commonly downregulated in glioblastomas.

Molecular Weight: 22 kDa

Gene ID: 117145

UniProt: Q5T1C6

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway