GRB14 antibody (AA 1-246)

Catalog No. ABIN7599149

The Rabbit Polyclonal anti-GRB14 antibody has been validated for WB, ELISA, IHC, IF, ICC and FACS. It is suitable to detect GRB14 in samples from Human.

Quick Overview for GRB14 antibody (AA 1-246) (ABIN7599149)

Target: GRB14 (Growth Factor Receptor-Bound Protein 14 (GRB14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GRB14 Antibody

Binding Specificity: AA 1-246

Purpose: Anti-GRB14 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GRB14 Antibody Picoband® (ABIN7599149). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GRB14 recombinant protein (Position: M1-Q246).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Daly, R. J., Sanderson, G. M., Janes, P. W., Sutherland, R. L. Cloning and characterization of GRB14, a novel member of the GRB7 gene family. J. Biol. Chem. 271: 12502-12510, 1996. 2. Depetris, R. S., Hu, J., Gimpelevich, I., Holt, L. J., Daly, R. J., Hubbard, S. R. Structural basis for inhibition of the insulin receptor by the adaptor protein Grb14. Molec. Cell 20: 325-333, 2005. 3. Dong, L. Q., Du, H., Porter, S. G., Kolakowski, L. F., Jr., Lee, A. V., Mandarino, L. J., Fan, J., Yee, D., Liu, F. Cloning, chromosome localization, expression, and characterization of an Src homology 2 and pleckstrin homology domain-containing insulin receptor binding protein hGrb10-gamma. J. Biol. Chem. 272: 29104-29112, 1997. Note: Erratum: J. Biol. Chem. 273: 4288 only, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GRB14

Target: GRB14 (Growth Factor Receptor-Bound Protein 14 (GRB14))

Alternative Name: GRB14

Background:

Synonyms: Endothelial cell-specific molecule 1, ESM-1, ESM1

Tissue Specificity: Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.

Background: Growth factor receptor-bound protein 14 is a protein that in humans is encoded by the GRB14 gene. The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants.

Molecular Weight: 61 kDa

Gene ID: 2888

UniProt: Q14449