NIT2 antibody (AA 1-249)

Catalog No. ABIN7599155

The Rabbit Polyclonal anti-NIT2 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect NIT2 in samples from Human, Mouse and Rat.

Quick Overview for NIT2 antibody (AA 1-249) (ABIN7599155)

Target: NIT2 (Nitrilase Family, Member 2 (NIT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NIT2 Antibody

Binding Specificity: AA 1-249

Purpose: Anti-NIT2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NIT2 Antibody Picoband® (ABIN7599155). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NIT2 recombinant protein (Position: M1-K249).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Asakura, M., Nakano, M., Hayashida, K., Fujii, H., Nakajima, M., Atsuda, K., Itoh, T., Fujiwara, R. Human nitrilase-like protein does not catalyze the hydrolysis of vildagliptin. Drug Metab. Pharmacokinet. 29: 463-469, 2014. 2. Chien, C.-H., Gao, Q.-Z., Cooper, A. J. L., Lyu, J.-H., Sheu, S.-Y. Structural insights into the catalytic active site and activity of human Nit2/omega-amidase: kinetic assay and molecular dynamics simulation. J. Biol. Chem. 287: 25715-25726, 2012. 3. Jaisson, S., Veiga-da-Cunha, M., Van Schaftingen, E. Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2. Biochimie 91: 1066-1071, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NIT2

Target: NIT2 (Nitrilase Family, Member 2 (NIT2))

Alternative Name: NIT2

Background:

Synonyms: Troponin T, fast skeletal muscle, TnTf, Beta-TnTF, Fast skeletal muscle troponin T, fTnT, TNNT3

Tissue Specificity: In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.

Background: NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.

Molecular Weight: 33 kDa

Gene ID: 56954