THO Complex 4 antibody (AA 1-257)

Catalog No. ABIN7599173

The Rabbit Polyclonal anti-THO Complex 4 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect THO Complex 4 in samples from Human, Mouse and Rat.

Quick Overview for THO Complex 4 antibody (AA 1-257) (ABIN7599173)

Target: THO Complex 4 (THOC4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THO Complex 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-THO Complex 4 Antibody

Binding Specificity: AA 1-257

Purpose: Anti-ALYREF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ALYREF Antibody Picoband® (ABIN7599173). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALYREF recombinant protein (Position: M1-S257).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bruhn, L., Munnerlyn, A., Grosschedl, R. ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCR-alpha enhancer function. Genes Dev. 11: 640-653, 1997. 2. Luo, M.-J., Zhou, Z., Magni, K., Christoforides, C., Rappsilber, J., Mann, M., Reed, R. Pre-mRNA splicing and mRNA export linked by interactions between UAP56 and Aly. Nature 413: 644-647, 2001. 3. Masuda, S., Das, R., Cheng, H., Hurt, E., Dorman, N., Reed, R. Recruitment of the human TREX complex to mRNA during splicing. Genes Dev. 19: 1512-1517, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for THO Complex 4

Target: THO Complex 4 (THOC4)

Alternative Name: ALYREF

Background:

Synonyms: Segment polarity protein dishevelled homolog DVL-3,Dishevelled-3,DSH homolog 3,DVL3,KIAA0208,

Background: Aly/REF export factor, also known as THO complex subunit 4 is a protein that in humans is encoded by the ALYREF gene. The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

Molecular Weight: 35 kDa

Gene ID: 10189

UniProt: Q86V81