ANAPC5 antibody (AA 1-266)

Catalog No. ABIN7599186

The Rabbit Polyclonal anti-ANAPC5 antibody (ABIN7599186) specifically detects ANAPC5 in WB, ELISA, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for ANAPC5 antibody (AA 1-266) (ABIN7599186)

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ANAPC5 Antibody

Binding Specificity: AA 1-266

Purpose: Anti-Apc5/ANAPC5 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Apc5/ANAPC5 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Apc5/ANAPC5 recombinant protein (Position: M1-D266).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ANAPC5

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Alternative Name: ANAPC5

Background:

Background: Anaphase-promoting complex subunit 5 is an enzyme that in humans is encoded by the ANAPC5 gene. This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

Gene Full Name: anaphase promoting complex subunit 5

Molecular Weight: 85 kDa

Gene ID: 51433

UniProt: Q9UJX4

Pathways: M Phase