SGTA antibody (AA 1-282)

Catalog No. ABIN7599212

The Rabbit Polyclonal anti-SGTA antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect SGTA in samples from Human, Mouse and Rat.

Quick Overview for SGTA antibody (AA 1-282) (ABIN7599212)

Target: SGTA (Small Glutamine-Rich Tetratricopeptide Repeat (TPR)-Containing, alpha (SGTA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGTA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SGTA Antibody

Binding Specificity: AA 1-282

Purpose: Anti-SGTA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SGTA Antibody Picoband® (ABIN7599212). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SGTA recombinant protein (Position: M1-Q282).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kordes, E., Savelyeva, L., Schwab, M., Rommelaere, J., Jauniaux, J.-C., Cziepluch, C. Isolation and characterization of human SGT and identification of homologues in Saccharomyces cerevisiae and Caenorhabditis elegans. Genomics 52: 90-94, 1998. 2. Shao, S., Rodrigo-Brenni, M. C., Kivlen, M. H., Hegde, R. S. Mechanistic basis for a molecular triage reaction. Science 355: 298-302, 2017. 3. Tobaben, S., Thakur, P., Fernandez-Chacon, R., Sudhof, T. C., Rettig, J., Stahl, B. A trimeric protein complex functions as a synaptic chaperone machine. Neuron 31: 987-999, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SGTA

Target: SGTA (Small Glutamine-Rich Tetratricopeptide Repeat (TPR)-Containing, alpha (SGTA))

Alternative Name: SGTA

Background:

Synonyms: SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2, UBA2, SAE2, UBLE1B, HRIHFB2115

Tissue Specificity: Duodenum, heart, lung and liver, but not thymus.

Background: Small glutamine-rich tetratricopeptide repeat-containing protein alpha is a protein that in humans is encoded by the SGTA gene. This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70- kDa heat shock cognate protein, however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.

Molecular Weight: 37 kDa

Gene ID: 6449

UniProt: O43765