Exonuclease 1 antibody (AA 1-294)

Catalog No. ABIN7599229

This Rabbit Polyclonal antibody specifically detects Exonuclease 1 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Exonuclease 1 antibody (AA 1-294) (ABIN7599229)

Target: Exonuclease 1 (EXO1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Exonuclease 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Exonuclease 1 Antibody

Binding Specificity: AA 1-294

Purpose: Anti-Exonuclease 1/EXO1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Exonuclease 1/EXO1 Antibody Picoband® (ABIN7599229). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Exonuclease 1/EXO1 recombinant protein (Position: M1-K294).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1.Zakharyevich K, Tang S, Ma Y, Hunter N (2012). "Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase". Cell. 149 (2): 334-47. 2. Wilson DM III, Carney JP, Coleman MA, Adamson AW, Christensen M, Lamerdin JE (September 1998). "Hex1: a new human Rad2 nuclease family member with homology to yeast exonuclease 1". Nucleic Acids Res. 26 (16): 3762-8. doi:10.1093/nar/26.16.3762. 3. Schmutte C, Marinescu RC, Sadoff MM, Guerrette S, Overhauser J, Fishel R (November 1998). "Human exonuclease I interacts with the mismatch repair protein hMSH2". Cancer Res. 58 (20): 4537-42.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Exonuclease 1

Target: Exonuclease 1 (EXO1)

Alternative Name: EXO1

Background:

Synonyms: Exodeoxyribonuclease 1, Exodeoxyribonuclease I, EXO I, Exonuclease I, Protein DHS1, EXO1, DHS1, YOR033C, OR26.23

Tissue Specificity: Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.

Background: Exonuclease 1?is an?enzyme?that in humans is encoded by the?EXO1?gene. It is mapped to 1q43. This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.

Molecular Weight: 120 kDa

Gene ID: 9156

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response