NEK7 antibody (AA 1-302)

Catalog No. ABIN7599245

The Rabbit Polyclonal anti-NEK7 antibody has been validated for WB, ELISA, IF, IP, ICC and FACS. It is suitable to detect NEK7 in samples from Human, Mouse and Rat.

Quick Overview for NEK7 antibody (AA 1-302) (ABIN7599245)

Target: NEK7

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NEK7 Antibody

Binding Specificity: AA 1-302

Purpose: Anti-NEK7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NEK7 Antibody Picoband® (ABIN7599245). Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NEK7 recombinant protein (Position: M1-S302). Human NEK7 shares 97.7% and 98% amino acid (aa) sequence identity with mouse and rat NEK7, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 2-4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. He, Y., Zeng, M. Y., Yang, D., Motro, B., Nunez, G. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 530: 354-357, 2016. 2. Kimura, M., Okano, Y. Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3. Cytogenet. Cell Genet. 94: 33-38, 2001. 3. Sharif, H., Wang, L., Wang, W. L., Magupalli, V. G., Andreeva, L., Qiao, Q., Hauenstein, A. V., Wu, Z., Nunez, G., Mao, Y., Wu, H. Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome. Nature 570: 338-343, 2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NEK7

Target: NEK7

Alternative Name: NEK7

Background:

Synonyms: NEK7, Serine/threonine-protein kinase Nek7, EC 2.7.11.1, Never in mitosis A-related kinase 7, NimA-related protein kinase 7

Background: NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.

Molecular Weight: 35 kDa

Gene ID: 140609

Pathways: Inflammasome