ATG4B antibody (AA 1-392)

Catalog No. ABIN7599346

This Rabbit Polyclonal antibody specifically detects ATG4B in WB, ELISA and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for ATG4B antibody (AA 1-392) (ABIN7599346)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ATG4B Antibody

Binding Specificity: AA 1-392

Purpose: Anti-ATG4B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ATG4B Antibody Picoband® (ABIN7599346). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATG4B recombinant protein (Position: M1-L392).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Agrotis, A., Pengo, N., Burden, J. J., Ketteler, R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15: 976-997, 2019. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/8/2019. 3. Hemelaar, J., Lelyveld, V. S., Kessler, B. M., Ploegh, H. L. A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L. J. Biol. Chem. 278: 51841-51850, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B

Background:

Synonyms: Glycerol kinase, GK, Glycerokinase, ATP:glycerol 3-phosphotransferase, GK

Tissue Specificity: Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.

Background: Cysteine protease ATG4B is an enzyme that in humans is encoded by the ATG4B gene. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 48 kDa

Gene ID: 23192

UniProt: Q9Y4P1

Pathways: Autophagy