ACTR2 antibody (AA 1-394)

Catalog No. ABIN7599349

This Rabbit Polyclonal antibody specifically detects ACTR2 in WB, IHC, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for ACTR2 antibody (AA 1-394) (ABIN7599349)

Target: ACTR2 (Actin-Related Protein 2 (ACTR2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-ACTR2 Antibody

Binding Specificity: AA 1-394

Purpose: Anti-Arp2/ACTR2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Arp2/ACTR2 Antibody Picoband® (ABIN7599349). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Arp2/ACTR2 recombinant protein (Position: M1-R394).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Caridi, C. P., D'Agostino, C., Ryu, T., Zapotoczny, G., Delabaere, L., Li, X., Khodaverdian, V. Y., Amaral, N., Lin, E., Rau, A. R., Chiolo, I. Nuclear F-actin and myosins drive relocalization of heterochromatic breaks. Nature 559: 54-60, 2018. 2. Hanisch, J., Kolm, R., Wozniczka, M., Bumann, D., Rottner, K., Stradal, T. E. B. Activation of a RhoA/myosin II-dependent but Arp2/3 complex-independent pathway facilitates Salmonella invasion. Cell Host Microbe 9: 273-285, 2011. 3. Li, P., Banjade, S., Cheng, H.-C., Kim, S., Chen, B., Guo, L., Llaguno, M., Hollingsworth, J. V., King, D. S., Banani, S. F., Russo, P. S., Jiang, Q.-X., Nixon, B. T., Rosen, M. K. Phase transitions in the assembly of multivalent signalling proteins. Nature 483: 336-340, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ACTR2

Target: ACTR2 (Actin-Related Protein 2 (ACTR2))

Alternative Name: ACTR2

Background:

Synonyms: Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDOB, ALDB

Tissue Specificity: Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.

Background: The specific function of this gene has not yet been determined, however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 45 kDa

Gene ID: 10097

UniProt: P61160

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization