TRIP13 antibody (AA 1-421)

Catalog No. ABIN7599379

This Rabbit Polyclonal antibody specifically detects TRIP13 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for TRIP13 antibody (AA 1-421) (ABIN7599379)

Target: TRIP13 (Thyroid Hormone Receptor Interactor 13 (TRIP13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIP13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TRIP13 Antibody

Binding Specificity: AA 1-421

Purpose: Anti-PCH2/TRIP13 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PCH2/TRIP13 Antibody Picoband® (ABIN7599379). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PCH2/TRIP13 recombinant protein (Position: M1-Q421).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alfieri, C., Chang, L., Barford, D. Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. Nature 559: 274-278, 2018. 2. Eytan, E., Wang, K., Miniowitz-Shemtov, S., Sitry-Shevah, D., Kaisari, S., Yen, T. J., Liu, S.-T., Hershko, A. Disassembly of mitotic checkpoint complexes by the joint action of the AAA-ATPase TRIP13 and p13(comet). Proc. Nat. Acad. Sci. 111: 12019-12024, 2014. 3. Gross, M. B. Personal Communication. Baltimore, Md. 6/25/2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRIP13

Target: TRIP13 (Thyroid Hormone Receptor Interactor 13 (TRIP13))

Alternative Name: TRIP13

Background:

Synonyms: Interleukin-34, IL-34, IL34, C16orf77

Tissue Specificity: Detected in the sinusoidal epithelium in the red pulp of spleen. Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Background: TRIP13 is a mammalian gene that encodes the thyroid receptor-interacting protein 13. This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.

Molecular Weight: 49 kDa

Gene ID: 9319

UniProt: Q15645