FLI1 antibody (AA 1-452)

Catalog No. ABIN7599409

This Rabbit Polyclonal antibody specifically detects FLI1 in WB, ELISA, IHC, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FLI1 antibody (AA 1-452) (ABIN7599409)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-FLI1 Antibody

Binding Specificity: AA 1-452

Purpose: Anti-FLI1 Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FLI1 Picoband® Antibody (ABIN7599409). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FLI1 recombinant protein (Position: M1-Y452).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Lin, P. P., Brody, R. I., Hamelin, A. C., Bradner, J. E., Healey, J. H., Ladanyi, M. Differential transactivation by alternative EWS-FLI1 fusion proteins correlates with clinical heterogeneity in Ewing's sarcoma. Cancer Res. 59: 1428-1432, 1999. 2. May, W. A., Gishizky, M. L., Lessnick, S. L., Lunsford, L. B., Lewis, B. C., Delattre, O., Zucman, J., Thomas, G., Denny, C. T. Ewing sarcoma 11,22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation. Proc. Nat. Acad. Sci. 90: 5752-5756, 1993. 3. Nakatani, F., Tanaka, K., Sakimura, R., Matsumoto, Y., Matsunobu, T., Li, X., Hanada, M., Okada, T., Iwamoto, Y. Identification of p21(WAF1/CIP1) as a target of EWS-Fli1 oncogenic fusion protein. J. Biol. Chem. 278: 15105-15115, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: FLI1

Background:

Synonyms: Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1

Tissue Specificity: Expressed in most tissues tested, including skin, lung, kidney, heart, testis and stomach.

Background: Friend leukemia integration 1 transcription factor (FLI1), also known as transcription factor ERGB, is a protein that in humans is encoded by the FLI1 gene, which is a proto-oncogene. It is mapped to 11q24.3. This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t (11,22) (q24,q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t (4,11) (q21,q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Molecular Weight: 54 kDa

Gene ID: 2313

UniProt: Q01543