GSDMD antibody (AA 1-484)

Catalog No. ABIN7599432

This anti-GSDMD antibody is a Mouse Monoclonal antibody detecting GSDMD in WB and IHC. Suitable for Human.

Quick Overview for GSDMD antibody (AA 1-484) (ABIN7599432)

Target: GSDMD (Gasdermin D (GSDMD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GSDMD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 3D5

Product Details anti-GSDMD Antibody

Binding Specificity: AA 1-484

Purpose: Anti-GSDMD Antibody Picoband® (monoclonal, 3D5)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GSDMD Antibody Picoband® (monoclonal, 3D5) (ABIN7599432). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GSDMD recombinant protein (Position: M1-H484).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Ruhl, S., Shkarina, K., Demarco, B., Heilig, R., Santos, J. C., Broz, P. ESCRT-dependent membrane repair negatively regulates pyroptosis downstream of GSDMD activation. Science 362: 956-960, 2018. 2. Shi, J., Zhao, Y., Wang, K., Shi, X., Wang, Y., Huang, H., Zhuang, Y., Cai, T., Wang, F., Shao, F. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 526: 660-665, 2015. 3. Zhu, Q., Zheng, M., Balakrishnan, A., Karki, R., Kanneganti, T.-D. Gasdermin D promotes AIM2 inflammasome activation and is required for host protection against Francisella novicida. J. Immun. 201: 3662-3668, 2018.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GSDMD

Target: GSDMD (Gasdermin D (GSDMD))

Alternative Name: GSDMD

Background:

Synonyms: "chromosomal protein, Nonhistone, HMG4 antibody|High mobility group (nonhistone chromosomal) protein 4 antibody|High mobility group box 3 antibody|High mobility group protein 2a antibody|High mobility group protein 4 antibody|High mobility group protein B3 antibody| High mobility group protein HMG4 antibody|HMG 4 antibody|HMG-2a antibody|HMG-4 antibody|HMG2A antibody|HMGB 3 antibody|HMGB3 antibody| HMGB3_HUMAN antibody|MGC90319 antibody|Non histone chromosomal protein antibody|Nonhistone chromosomal protein HMG4 antibody"

Tissue Specificity: Expressed predominantly in placenta.

Background: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Molecular Weight: 53 kDa

Gene ID: 79792

UniProt: P57764

Pathways: Inflammasome