TRIM25 antibody (AA 1-590)

Catalog No. ABIN7599491

The Rabbit Polyclonal anti-TRIM25 antibody is suitable to detect TRIM25 in samples from Mouse and Rat. It has been validated for WB, ELISA, IHC, IF, ICC and FACS.

Quick Overview for TRIM25 antibody (AA 1-590) (ABIN7599491)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-TRIM25 Antibody

Binding Specificity: AA 1-590

Purpose: Anti-TRIM25/EFP Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRIM25/EFP Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse TRIM25/EFP recombinant protein (Position: M1-H590).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse, Rat ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: Trim25

Background:

Background: Tripartite motif-containing protein 25 is a protein that in humans is encoded by the TRIM25 gene. It is mapped to 17q22. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Gene Full Name: tripartite motif-containing 25

Molecular Weight: 71 kDa

Gene ID: 217069

UniProt: Q61510