FBXW8 antibody (AA 1-598)

Catalog No. ABIN7599492

This anti-FBXW8 antibody is a Rabbit Polyclonal antibody detecting FBXW8 in WB and ELISA. Suitable for Human and Rat.

Quick Overview for FBXW8 antibody (AA 1-598) (ABIN7599492)

Target: FBXW8 (F-Box and WD Repeat Domain Containing 8 (FBXW8))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FBXW8 Antibody

Binding Specificity: AA 1-598

Purpose: Anti-FBXW8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-FBXW8 Antibody Picoband® (ABIN7599492). Tested in WB, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FBXW8 recombinant protein (Position: M1-V598). Human FBXW8 shares 78.5% and 77.4% amino acid (aa) sequence identity with mouse and rat FBXW8, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
ELISA, 0.1-0.5 μg/mL
1. Arai, T., Kasper, J. S., Skaar, J. R., Ali, S. H., Takahashi, C., DeCaprio, J. A. Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis. Proc. Nat. Acad. Sci. 100: 9855-9860, 2003. 2. Dias, D. C., Dolios, G., Wang, R., Pan, Z.-Q. CUL7: A DOC domain-containing cullin selectively binds Skp1-Fbx29 to form an SCF-like complex. Proc. Nat. Acad. Sci. 99: 16601-16606, 2002. 3. Jin, J., Cardozo, T., Lovering, R. C., Elledge, S. J., Pagano, M., Harper, J. W. Systematic analysis and nomenclature of mammalian F-box proteins. Genes Dev. 18: 2573-2580, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FBXW8

Target: FBXW8 (F-Box and WD Repeat Domain Containing 8 (FBXW8))

Alternative Name: FBXW8

Background:

Synonyms: FBXW8, FBW6, FBW8, FBX29, FBXO29, FBXW6, F-box/WD repeat-containing protein 8, F-box and WD-40 domain-containing protein 8, F-box only protein 29

Background: This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

Molecular Weight: 67 kDa

Gene ID: 26259

UniProt: Q8N3Y1