T-Box 18 antibody (AA 1-607)

Catalog No. ABIN7599494

The Rabbit Polyclonal anti-T-Box 18 antibody has been validated for ELISA, WB, IHC, IF and FACS. It is suitable to detect T-Box 18 in samples from Human, Mouse and Rat.

Quick Overview for T-Box 18 antibody (AA 1-607) (ABIN7599494)

Target: T-Box 18 (TBX18)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Box 18 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-T-Box 18 Antibody

Binding Specificity: AA 1-607

Purpose: Anti-TBX18 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TBX18 Antibody Picoband® (ABIN7599494). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TBX18 recombinant protein (Position: M1-V607).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Airik, R., Bussen, M., Singh, M. K., Petry, M., Kispert, A. Tbx18 regulates the development of the ureteral mesenchyme. J. Clin. Invest. 116: 663-674, 2006. 2. Bussen, M, Petry, M., Schuster-Gossler, K., Leitges, M., Gossler, A., Kispert, A. The T-box transcription factor Tbx18 maintains the separation of anterior and posterior somite compartments. Genes Dev. 18: 1209-1221, 2004. 3. Cai, C.-L., Martin, J. C., Sun, Y., Cui, L., Wang, L., Ouyang, K., Yang, L., Bu, L., Liang, X., Zhang, X., Stallcup, W. B., Denton, C. P., McCulloch, A., Chen, J., Evans, S. M. A myocardial lineage derives from Tbx18 epicardial cells. Nature 454: 104-108, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for T-Box 18

Target: T-Box 18 (TBX18)

Alternative Name: TBX18

Background:

Synonyms: Aspartate--tRNA ligase, mitochondrial, Aspartyl-tRNA synthetase, AspRS, DARS2

Tissue Specificity: Detected in brain. Detected at very much lower levels in heart, lung, placenta and skeletal muscle. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Background: This genes codes for a member of an evolutionarily conserved family of transcription factors that plays a crucial role in embryonic development. The family is characterized by the presence of the DNA-binding T-box domain and is divided into five sub-families based on sequence conservation in this domain. The encoded protein belongs to the vertebrate specific Tbx1 sub-family. The protein acts as a transcriptional repressor by antagonizing transcriptional activators in the T-box family. The protein forms homo- or heterodimers with other transcription factors of the T-box family or other transcription factors.

Molecular Weight: 70 kDa

Gene ID: 9096

UniProt: O95935

Pathways: Sensory Perception of Sound