SYK antibody (AA 10-635)

Catalog No. ABIN7599608

The Rabbit Polyclonal anti-SYK antibody has been validated for WB, ELISA and FACS. It is suitable to detect SYK in samples from Human, Mouse and Rat.

Quick Overview for SYK antibody (AA 10-635) (ABIN7599608)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SYK Antibody

Binding Specificity: AA 10-635

Purpose: Anti-SYK Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SYK Antibody Picoband® (ABIN7599608). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SYK recombinant protein (Position: A10-N635).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Colucci, F., Schweighoffer, E., Tomasello, E., Turner, M., Ortaldo, J. R., Vivier, E., Tybulewicz, V. L. J., Di Santo, J. P. Natural cytotoxicity uncoupled from the Syk and ZAP-70 intracellular kinases. Nature Immun. 3: 288-294, 2002. 2. Coopman, P. J. P., Do, M. T. H., Barth, M., Bowden, E. T., Hayes, A. J., Basyuk, E., Blancato, J. K., Vezza, P. R., McLeskey, S. W., Mangeat, P. H., Mueller, S. C. The Syk tyrosine kinase suppresses malignant growth of human breast cancer cells. Nature 406: 742-747, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: SYK

Background:

Synonyms: Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk, SYK

Tissue Specificity: Widely expressed in hematopoietic cells. Expressed in neutrophils. Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells.

Background: Spleen tyrosine kinase, also known as Syk, is an enzyme which in humans is encoded by the SYK gene. It is mapped to 9q22.2. This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 72 kDa

Gene ID: 6850

UniProt: P43405

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling