CD69 antibody (AA 101-148)

Catalog No. ABIN7599623

The Rabbit Polyclonal anti-CD69 antibody has been validated for FACS, WB, IHC, ELISA, IF and ICC. It is suitable to detect CD69 in samples from Human, Mouse and Rat.

Quick Overview for CD69 antibody (AA 101-148) (ABIN7599623)

Target: CD69

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD69 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CD69 Antibody

Binding Specificity: AA 101-148

Purpose: Anti-CD69 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD69 Antibody Picoband® (ABIN7599623). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD69 recombinant protein (Position: T101-E148).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cambiaggi, C., Scupoli, M. T., Cestari, T., Gerosa, F., Carra, G., Tridente, G., Accolla, R. S. Constitutive expression of CD69 in interspecies T-cell hybrids and locus assignment to human chromosome 12. Immunogenetics 36: 117-120, 1992. 2. Esplugues, E., Sancho, D., Vega-Ramos, J., Martinez-A, C., Syrbe, U., Hamann, A., Engel, P., Sanchez-Madrid, F., Lauzurica, P. Enhanced antitumor immunity in mice deficient in CD69. J. Exp. Med. 197: 1093-1106, 2003. 3. Sancho, D., Gomez, M., Viedma, F., Esplugues, E., Gordon-Alonso, M., Garcia-Lopez, M. A., de la Fuente, H., Martinez-A, C., Lauzurica, P., Sanchez-Madrid, F. CD69 downregulates autoimmune reactivity through active transforming growth factor-beta production in collagen-induced arthritis. J. Clin. Invest. 112: 872-882, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD69

Target: CD69

Alternative Name: CD69

Background:

Synonyms: Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp

Tissue Specificity: Plasma.

Background: This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets.

Molecular Weight: 22 kDa

Gene ID: 969

UniProt: Q07108