DIAPH1 antibody (AA 1039-1153)

Catalog No. ABIN7599652

This Rabbit Polyclonal antibody specifically detects DIAPH1 in WB, IF, ELISA, ICC and FACS. It exhibits reactivity toward Human and Monkey.

Quick Overview for DIAPH1 antibody (AA 1039-1153) (ABIN7599652)

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIAPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-DIAPH1 Antibody

Binding Specificity: AA 1039-1153

Purpose: Anti-DIAPH1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DIAPH1 Antibody Picoband® (ABIN7599652). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DIAPH1 recombinant protein (Position: D1039-Q1153).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Al-Maawali, A., Barry, B. J., Rajab, A., El-Quessny, M., Seman, A., Coury, S. N., Barkovich, A. J., Yang, E., Walsh, C. A., Mochida, G. H., Stoler, J. M. Novel loss-of-function variants in DIAPH1 associated with syndromic microcephaly, blindness, and early onset seizures. Am. J. Med. Genet. 170A: 435-440, 2016. 2. Carreira, S., Goodall, J., Denat, L., Rodriguez, M., Nuciforo, P., Hoek, K. S., Testori, A., Larue, L., Goding, C. R. Mitf regulation of Dia1 controls melanoma proliferation and invasiveness. Genes Dev. 20: 3426-3439, 2006. 3. Ercan-Sencicek, A. G., Jambi, S., Franjic, D., Nishimura, S., Li, M., El-Fishawy, P., Morgan, T. M., Sanders, S. J., Bilguvar, K., Suri, M., Johnson, M. H., Gupta, A. R., Yuksel, Z., Mane, S., Grigorenko, E., Picciotto, M., Alberts, A. S., Gunel, M., Sestan, N., State, M. W. Homozygous loss of DIAPH1 is a novel cause of microcephaly in humans. Europ. J. Hum. Genet. 23: 165-172, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DIAPH1

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Alternative Name: DIAPH1

Background:

Synonyms: Adrenocorticotropic hormone receptor,ACTH receptor,ACTH-R,Adrenocorticotropin receptor,Melanocortin receptor 2,MC2-R,MC2R,ACTHR,

Tissue Specificity: Melanocytes and corticoadrenal tissue.

Background: Protein diaphanous homolog 1 is a protein that in humans is encoded by the DIAPH1 gene. This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molecular Weight: 150 kDa

Gene ID: 1729

UniProt: O60610

Pathways: Sensory Perception of Sound