LEPREL1 antibody (AA 106-494)

Catalog No. ABIN7599671

The Rabbit Polyclonal anti-LEPREL1 antibody has been validated for WB, ELISA, IHC, ICC, FACS and IF. It is suitable to detect LEPREL1 in samples from Human, Mouse and Rat.

Quick Overview for LEPREL1 antibody (AA 106-494) (ABIN7599671)

Target: LEPREL1 (Leprecan Like Protein 1 (LEPREL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEPREL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-LEPREL1 Antibody

Binding Specificity: AA 106-494

Purpose: Anti-P3H2 Antibody Picoband®

Characteristics: Anti-P3H2 Antibody Picoband® (ABIN7599671). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human P3H2 recombinant protein (Position: E106-R494). Human P3H2 shares 88.9% and 89.5% amino acid (aa) sequence identity with mouse and rat P3H2, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry, 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 10/18/2011. 2. Guo, H., Tong, P., Peng, Y., Wang, T., Liu, Y., Chen, J., Li, Y., Tian, Q., Hu, Y., Zheng, Y., Xiao, L., Xiong, W., Pan, Q., Hu, Z., Xia, K. Homozygous loss-of-function mutation of the LEPREL1 gene causes severe non-syndromic high myopia with early-onset cataract. Clin. Genet. 86: 575-579, 2014. 3. Jarnum, S., Kjellman, C., Darabi, A., Nilsson, I., Edvardsen, K., Aman, P. LEPREL1, a novel ER and Golgi resident member of the Leprecan family. Biochem. Biophys. Res. Commun. 317: 342-351, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LEPREL1

Target: LEPREL1 (Leprecan Like Protein 1 (LEPREL1))

Alternative Name: P3H2

Background: This gene encodes a member of the prolyl 3-hydroxylase subfamily of 2-oxo-glutarate-dependent dioxygenases. These enzymes play a critical role in collagen chain assembly, stability and cross-linking by catalyzing post-translational 3-hydroxylation of proline residues. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 81 kDa

Gene ID: 55214