RAB3GAP1 antibody (AA 109-810)

Catalog No. ABIN7599702

Product Details anti-RAB3GAP1 Antibody

Target: RAB3GAP1 (RAB3 GTPase Activating Protein Subunit 1 (Catalytic) (RAB3GAP1))

Binding Specificity: AA 109-810

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB3GAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Anti-RAB3GAP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAB3GAP1 Antibody Picoband® (ABIN7599702). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RAB3GAP1 recombinant protein (Position: N109-Q810).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abdel-Hamid, M. S., Abdel-Ghafar, S. F., Ismail, S. R., Desouky, L. M., Issa, M. Y., Effat, L. K., Zaki, M. S. Micro and Martsolf syndromes in 34 new patients: refining the phenotypic spectrum and further molecular insights. Clin. Genet. 98: 445-456, 2020. 2. Abdel-Salam, G. M. H., Hassan, N. A., Kayed, H. F., Aligianis, I. A. Phenotypic variability in Micro syndrome: report of new cases. Genet. Counsel. 18: 423-435, 2007. 3. Aligianis, I. A., Johnson, C. A., Gissen, P., Chen, D., Hampshire, D., Hoffmann, K., Maina, E. N., Morgan, N. V., Tee, L., Morton, J., Ainsworth, J. R., Horn, D., and 20 others. Mutations of the catalytic subunit of RAB3GAP cause Warburg Micro syndrome. Nature Genet. 37: 221-223, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RAB3GAP1

Target: RAB3GAP1 (RAB3 GTPase Activating Protein Subunit 1 (Catalytic) (RAB3GAP1))

Alternative Name: RAB3GAP1 (RAB3GAP1 Products)

Background:

Synonyms: Ras GTPase-activating-like protein IQGAP2, IQGAP2

Tissue Specificity: Widely expressed. Highly expressed in ovary followed by spleen, thymus, prostate, testes and large intestine. Weakly expressed in small intestine.

Background: Rab3 GTPase-activating protein catalytic subunit is an enzyme that in humans is encoded by the RAB3GAP1 gene. This gene encodes the catalytic subunit of a Rab GTPase activating protein. The encoded protein forms a heterodimer with a non-catalytic subunit to specifically regulate the activity of members of the Rab3 subfamily of small G proteins. This protein mediates the hydrolysis of GTP bound Rab3 to the GDP bound form. Mutations in this gene are associated with Warburg micro syndrome. Alternate splicing results in multiple transcript variants.

Molecular Weight: 130 kDa

Gene ID: 22930

UniProt: Q15042