IL3RA antibody (AA 111-378)

Catalog No. ABIN7599742

This Rabbit Polyclonal antibody specifically detects IL3RA in FACS, WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for IL3RA antibody (AA 111-378) (ABIN7599742)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL3RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IL3RA Antibody

Binding Specificity: AA 111-378

Purpose: Anti-IL3RA/CD123 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IL3RA/ Antibody Picoband® (ABIN7599742). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IL3RA/CD123 recombinant protein (Position: T111-T378).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Itoh, N., Yonehara, S., Schreurs, J., Gorman, D. M., Maruyama, K., Ishii, A., Yahara, I., Arai, K., Miyajima, A. Cloning of an interleukin-3 receptor gene: a member of a distinct receptor gene family. Science 247: 324-327, 1990. 2. Kitamura, T., Sato, N., Arai, K., Miyajima, A. Expression cloning of the human IL-3 receptor cDNA reveals a shared beta-subunit for the human IL-3 and GM-CSF receptors. Cell 66: 1165-1174, 1991. 3. Kosugi, H., Nakagawa, Y., Hotta, T., Saito, H., Miyajima, A., Arai, K., Yokota, T. Structure of the gene encoding the alpha subunit of the human interleukin 3 receptor. Biochem. Biophys. Res. Commun. 208: 360-367, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: IL3RA

Background:

Synonyms: Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, CD123, IL3RA, IL3R

Tissue Specificity: Brain.

Background: Interleukin 3 receptor, alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a human gene. It is mapped to Xp22.33 and Yp11.2. The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.

Molecular Weight: 60 kDa

Gene ID: 3563

UniProt: P26951

Pathways: JAK-STAT Signaling