CDK12 antibody (AA 1147-1439)

Catalog No. ABIN7599764

The Rabbit Polyclonal anti-CDK12 antibody has been validated for ELISA, WB, IHC, IP, IF, FACS and ICC. It is suitable to detect CDK12 in samples from Human.

Quick Overview for CDK12 antibody (AA 1147-1439) (ABIN7599764)

Target: CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-CDK12 Antibody

Binding Specificity: AA 1147-1439

Purpose: Anti-CDK12 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CDK12 Antibody Picoband® (ABIN7599764). Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CDK12 recombinant protein (Position: Q1147-N1439).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Blazek, D., Kohoutek, J., Bartholomeeusen, K., Johansen, E., Hulinkova, P., Luo, Z., Cimermancic, P., Ule, J., Peterlin, B. M. The cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes. Genes Dev. 25: 2158-2172, 2011. 2. Cheng, S.-W. G., Kuzyk, M. A., Moradian, A., Ichu, T.-A., Chang, V. C.-D., Tien, J. F., Vollett, S. E., Griffith, M., Marra, M. A., Morin, G. B. Interaction of cyclin-dependent kinase 12/CrkRS with cyclin K1 is required for the phosphorylation of the C-terminal domain of RNA polymerase II. Molec. Cell. Biol. 32: 4691-4704, 2012. 3. Dubbury, S. J., Boutz, P. L., Sharp, P. A. CDK12 regulates DNA repair genes by suppressing intronic polyadenylation. Nature 564: 141-145, 2018.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CDK12

Target: CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))

Alternative Name: CDK12

Background:

Synonyms: Calpain-2 catalytic subunit,3.4.22.53,Calcium-activated neutral proteinase 2,CANP 2,Calpain M-type,Calpain large polypeptide L2,Calpain-2 large subunit,Millimolar-calpain,M-calpain,CAPN2,CANPL2,

Tissue Specificity: Ubiquitous.

Background: CDK12 cyclin-dependent kinase 12 is a protein kinase that in humans is encoded by the CDK12 gene. This enzyme is a member of cyclin-dependent kinase protein family. CDK12 forms protein complexes with cyclin K (CCNK). The CDK12-CCNK complex acts at the level of RNA polymerase II (RNAPII) to regulate expression of long complex genes, several of which have roles in DNA maintenance and repair.

Molecular Weight: 205 kDa

Gene ID: 51755