HSF4 antibody (AA 118-277)

Catalog No. ABIN7599790

This anti-HSF4 antibody is a Rabbit Polyclonal antibody detecting HSF4 in WB and ELISA. Suitable for Human.

Quick Overview for HSF4 antibody (AA 118-277) (ABIN7599790)

Target: HSF4 (Heat Shock Transcription Factor 4 (HSF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HSF4 Antibody

Binding Specificity: AA 118-277

Purpose: Anti-HSF4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HSF4 Antibody Picoband® (ABIN7599790). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HSF4 recombinant protein (Position: R118-L277).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bu L, Jin Y, Shi Y, Chu R, Ban A, Eiberg H, Andres L, Jiang H, Zheng G, Qian M, Cui B, Xia Y, Liu J, Hu L, Zhao G, Hayden MR, Kong X (Jun 2002). "Mutant DNA-binding domain of HSF4 is associated with autosomal dominant lamellar and Marner cataract". Nat Genet 31 (3): 276-8. 2. Nakai A, Tanabe M, Kawazoe Y, Inazawa J, Morimoto RI, Nagata K (Jan 1997). "HSF4, a new member of the human heat shock factor family which lacks properties of a transcriptional activator". Mol Cell Biol 17 (1): 469-81. 3. Tanabe M, Sasai N, Nagata K, Liu XD, Liu PC, Thiele DJ, Nakai A (Nov 1999). "The mammalian HSF4 gene generates both an activator and a repressor of heat shock genes by alternative splicing". J Biol Chem 274(39): 27845-56.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HSF4

Target: HSF4 (Heat Shock Transcription Factor 4 (HSF4))

Alternative Name: HSF4

Background:

Synonyms: Interleukin-25, IL-25, Interleukin-17E, IL-17E, IL25, IL17E, UNQ3120/PRO10272

Tissue Specificity: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.

Background: Heat shock factor protein 4 is a protein that in humans is encoded by the HSF4 gene. It is mapped to 16q21. Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.

Molecular Weight: 53 kDa

Gene ID: 3299

Pathways: MAPK Signaling