BCL3 antibody (AA 118-307)

Catalog No. ABIN7599792

The Rabbit Polyclonal anti-BCL3 antibody has been validated for WB, ELISA and FACS. It is suitable to detect BCL3 in samples from Human, Mouse and Rat.

Quick Overview for BCL3 antibody (AA 118-307) (ABIN7599792)

Target: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-BCL3 Antibody

Binding Specificity: AA 118-307

Purpose: Anti-BCL3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BCL3 Antibody Picoband® (ABIN7599792). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BCL3 recombinant protein (Position: E118-Y307).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Wulczyn FG, Naumann M, Scheidereit C (Sep 1992). "Candidate proto-oncogene bcl-3 encodes a subunit-specific inhibitor of transcription factor NF-kappa B". Nature 358 (6387): 597-9. 2. Ohno H, Takimoto G, McKeithan TW (May 1990). "The candidate proto-oncogene bcl-3 is related to genes implicated in cell lineage determination and cell cycle control". Cell 60 (6): 991-7. 3. McKeithan, T. W., Rowley, J. D., Shows, T. B., Diaz, M. O.Cloning of the chromosome translocation breakpoint junction of the t(14,19) in chronic lymphocytic leukemia.Proc. Nat. Acad. Sci. 84: 9257-9260, 1987.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for BCL3

Target: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Alternative Name: BCL3

Background:

Synonyms: CD3 antigen, epsilon polypeptide, CD3e molecule, Cd3e, Cd3e_predicted, rCG_58471

Background: B-cell lymphoma 3-encoded protein is a protein that in humans is encoded by the BCL3 gene. This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. The BCL3 gene is mapped to human chromosome 19. This protein functions as a transcriptional coactivator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B.

Molecular Weight: 60 kDa

Gene ID: 602

UniProt: P20749

Pathways: NF-kappaB Signaling, Maintenance of Protein Location, Protein targeting to Nucleus