BCL3 antibody (AA 118-420)

Catalog No. ABIN7599795

The Rabbit Polyclonal anti-BCL3 antibody has been validated for WB, ELISA, IF, FACS and ICC. It is suitable to detect BCL3 in samples from Human, Mouse and Rat.

Quick Overview for BCL3 antibody (AA 118-420) (ABIN7599795)

Target: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-BCL3 Antibody

Binding Specificity: AA 118-420

Purpose: Anti-BCL3 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-BCL3 Antibody Picoband® (ABIN7599795). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PELI2 recombinant protein (Position: D118-D420). Human PELI2 shares 97.4% amino acid (aa) sequence identity with mouse?PELI2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bhatia, K., Huppi, K., McKeithan, T., Siwarski, D., Mushinski, J. F., Magrath, I. Mouse bcl-3: cDNA structure, mapping and stage-dependent expression in B lymphocytes. Oncogene 6: 1569-1573, 1991. 2. Carmody, R. J., Ruan, Q., Palmer, S., Hilliard, B., Chen, Y. H. Negative regulation of Toll-like receptor signaling by NF-kappa-B p50 ubiquitination blockade. Science 317: 675-678, 2007. 3. Franzoso, G., Bours, V., Park, S., Tomita-Yamaguchi, M., Kelly, K., Siebenlist, U. The candidate oncoprotein Bcl-3 is an antagonist of p50/NF-kappa-B-mediated inhibition. Nature 359: 339-342, 1992.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for BCL3

Target: BCL3 (B-Cell CLL/lymphoma 3 (BCL3))

Alternative Name: BCL3

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: B-cell lymphoma 3-encoded protein is a protein that in humans is encoded by the BCL3 gene. This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B.

Molecular Weight: 60 kDa

Gene ID: 602

UniProt: P20749

Pathways: NF-kappaB Signaling, Maintenance of Protein Location, Protein targeting to Nucleus