RNF212 antibody (AA 12-271)

Catalog No. ABIN7599812

This Rabbit Polyclonal antibody specifically detects RNF212 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RNF212 antibody (AA 12-271) (ABIN7599812)

Target: RNF212 (Ring Finger Protein 212 (RNF212))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF212 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RNF212 Antibody

Binding Specificity: AA 12-271

Purpose: Anti-RNF212 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-R Antibody Picoband® (ABIN7599812). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF212 recombinant protein (Position: Q12-E271).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ahuja, J. S., Sandhu, R., Mainpal, R., Lawson, C., Henley, H., Hunt, P. A., Yanowitz, J. L., Borner, G. V. Control of meiotic pairing and recombination by chromosomally tethered 26S proteasome. Science 355: 408-411, 2017. 2. Jantsch, V., Pasierbek, P., Mueller, M. M., Schweizer, D., Jantsch, M., Loidl, J. Targeted gene knockout reveals a role in meiotic recombination for ZHP-3, a Zip3-related protein in Caenorhabditis elegans. Molec. Cell. Biol. 24: 7998-8006, 2004. 3. Kong, A., Thorleifsson, G., Stefansson, H., Masson, G., Helgason, A., Gudbjartsson, D. F., Jonsdottir, G. M., Gudjonsson, S. A., Sverrisson, S., Thorlacius, T., Jonasdottir, A., Hardarson, G. A., Palsson, S. T., Frigge, M. L., Gulcher, J. R., Thorsteinsdottir, U., Stefansson, K. Sequence variants in the RNF212 gene associate with genome-wide recombination rate. Science 319: 1398-1401, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RNF212

Target: RNF212 (Ring Finger Protein 212 (RNF212))

Alternative Name: RNF212

Background:

Synonyms: Protein eva-1 homolog A, Protein FAM176A, Transmembrane protein 166, EVA1A, FAM176A, TMEM166, SP24

Tissue Specificity: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Background: This gene encodes a RING finger protein that may function as a ubiquitin ligase. The encoded protein may be involved in meiotic recombination. This gene is located within a linkage disequilibrium block and polymorphisms in this gene may influence recombination rates. Alternate splicing results in multiple transcript variants.

Molecular Weight: 22 kDa

Gene ID: 285498

UniProt: Q495C1