HPD antibody (AA 12-357)

Catalog No. ABIN7599818

This Rabbit Polyclonal antibody specifically detects HPD in WB, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HPD antibody (AA 12-357) (ABIN7599818)

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HPD Antibody

Binding Specificity: AA 12-357

Purpose: Anti-HPD Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HPD Antibody Picoband® (ABIN7599818). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HPD recombinant protein (Position: E12-Q357).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Awata, H., Endo, F., Matsuda, I. Structure of the human 4-hydroxyphenylpyruvic acid dioxygenase gene (HPD). Genomics 23: 534-539, 1994. 2. Borden, M., Holm, J., Leslie, J., Sweetman, L., Nyhan, W. L., Fleisher, L., Nadler, H., Lewis, D., Scott, C. R. Hawkinsinuria in two families. Am. J. Med. Genet. 44: 52-56, 1992. 3. Cerone, R., Holme, E., Schiaffino, M. C., Caruso, U., Maritano, L., Romano, C. Tyrosinemia type III: diagnosis and ten-year follow-up. Acta Paediat. 86: 1013-1015, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HPD

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Alternative Name: HPD

Background:

Synonyms: Elongator complex protein 3, Help3, ELP3

Tissue Specificity: Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level). Also expressed in jejunum and duodenum.

Background: The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 45 kDa

Gene ID: 3242

UniProt: P32754