HJURP antibody (AA 13-574)

Catalog No. ABIN7599943

This anti-HJURP antibody is a Rabbit Polyclonal antibody detecting HJURP in WB, ELISA and FACS. Suitable for Mouse and Rat.

Quick Overview for HJURP antibody (AA 13-574) (ABIN7599943)

Target: HJURP (Holliday Junction Recognition Protein (HJURP))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HJURP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-HJURP Antibody

Binding Specificity: AA 13-574

Purpose: Anti-Hjurp Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Hjurp Antibody Picoband® (ABIN7599943). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Hjurp recombinant protein (Position: Q13-T574).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/16/2009. 2. Kato, T., Sato, N., Hayama, S., Yamabuki, T., Ito, T., Miyamoto, M., Kondo, S., Nakamura, Y., Daigo, Y. Activation of Holliday junction-recognizing protein involved in the chromosomal stability and immortality of cancer cells. Cancer Res. 67: 8544-8553, 2007. 3. Nardi, I. K., Zasadzinska, E., Stellfox, M. E., Knippler, C. M., Foltz, D. R. Licensing of centromeric chromatin assembly through the Mis18-alpha-Mis18-beta heterotetramer. Molec. Cell 61: 774-787, 2016.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HJURP

Target: HJURP (Holliday Junction Recognition Protein (HJURP))

Alternative Name: Hjurp

Background:

Synonyms: AF4/FMR2 family member 4,ALL1-fused gene from chromosome 5q31 protein,Protein AF-5q31,Major CDK9 elongation factor-associated protein,AFF4,AF5Q31, MCEF,HSPC092,

Tissue Specificity: Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain. .

Background: Holliday junction recognition protein is a protein in humans that is encoded by the HJURP gene. HJURP (holliday junction recognition protein) is a protein coding gene. Diseases associated with HJURP include fibrillary astrocytoma. Among its related pathways are DNA damage and chromosome maintenance. Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. ly binds holliday junctions.

Molecular Weight: 84 kDa

Gene ID: 381280