GORASP1 antibody (AA 130-440)

Catalog No. ABIN7599953

This Rabbit Polyclonal antibody specifically detects GORASP1 in WB, IF, ELISA, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for GORASP1 antibody (AA 130-440) (ABIN7599953)

Target: GORASP1 (Golgi Reassembly Stacking Protein 1, 65kDa (GORASP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GORASP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-GORASP1 Antibody

Binding Specificity: AA 130-440

Purpose: Anti-GRASP65/GORASP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GRASP65/GORASP1 Antibody Picoband® (ABIN7599953). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GRASP65/GORASP1 recombinant protein (Position: R130-E440).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barr, F. A., Nakamura, N., Warren, G. Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae. EMBO J. 17: 3258-3268, 1998. 2. Lane, J. D, Lucocq, J., Pryde, J., Barr, F. A., Woodman, P. G., Allan, V. J., Lowe, M. Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis. J. Cell Biol. 156: 495-509, 2002. 3. Protopopov, A., Kashuba, V., Zabarovska, V. I., Muravenko, O. V., Lerman, M. I., Klein, G., Zabarovsky, E. R. An integrated physical and gene map of the 3.5-Mb chromosome 3p21.3 (AP20) region implicated in major human epithelial malignancies. Cancer Res. 63: 404-412, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GORASP1

Target: GORASP1 (Golgi Reassembly Stacking Protein 1, 65kDa (GORASP1))

Alternative Name: GORASP1

Background:

Synonyms: Troponin T, fast skeletal muscle, TnTf, Beta-TnTF, Fast skeletal muscle troponin T, fTnT, TNNT3

Tissue Specificity: In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.

Background: Golgi reassembly-stacking protein of 65 kDa (GRASP65) also known as Golgi reassembly-stacking protein 1 (GORASP1) is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene.

Molecular Weight: 65 kDa

Gene ID: 64689

Pathways: SARS-CoV-2 Protein Interactome