AGLU antibody (AA 131-2697)

Catalog No. ABIN7599961

Product Details anti-AGLU Antibody

Target: AGLU (alpha-Glucosidase (AGLU))

Binding Specificity: AA 131-2697

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGLU antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Anti-MGAM Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MGAM Antibody Picoband® (ABIN7599961). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MGAM recombinant protein (Position: Y131-I2697).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Naim, H. Y., Sterchi, E. E., Lentze, M. J. Structure, biosynthesis, and glycosylation of human small intestinal maltase-glucoamylase. J. Biol. Chem. 263: 19709-19717, 1988. 2. Nichols, B. L., Avery, S., Sen, P., Swallow, D. M., Hahn, D., Sterchi, E. The maltase-glucoamylase gene: common ancestry to sucrase-isomaltase with complementary starch digestion activities. Proc. Nat. Acad. Sci. 100: 1432-1437, 2003. 3. Nichols, B. L., Eldering, J., Avery, S., Hahn, D., Quaroni, A., Sterchi, E. Human small intestinal maltase-glucoamylase cDNA cloning: homology to sucrase-isomaltase. J. Biol. Chem. 273: 3076-3081, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for AGLU

Target: AGLU (alpha-Glucosidase (AGLU))

Alternative Name: MGAM (AGLU Products)

Background:

Synonyms: Mesoderm posterior protein 1, Class C basic helix-loop-helix protein 5, bHLHc5, MESP1, BHLHC5

Tissue Specificity: Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. .

Background: Maltase-glucoamylase, intestinal is an enzyme that in humans is encoded by the MGAM gene. This gene encodes maltase-glucoamylase, which is a brush border membrane enzyme that plays a role in the final steps of digestion of starch. The protein has two catalytic sites identical to those of sucrase-isomaltase, but the proteins are only 59 % homologous. Both are members of glycosyl hydrolase family 31, which has a variety of substrate specificities.

Molecular Weight: 240 kDa

Gene ID: 8972

UniProt: O43451

Pathways: Cellular Glucan Metabolic Process