FHL1 antibody (AA 133-179)

Catalog No. ABIN7599973

This anti-FHL1 antibody is a Rabbit Polyclonal antibody detecting FHL1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for FHL1 antibody (AA 133-179) (ABIN7599973)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FHL1 Antibody

Binding Specificity: AA 133-179

Purpose: Anti-FHL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FHL1 Antibody Picoband® (ABIN7599973). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FHL1 recombinant protein (Position: K133-Q179).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Brown, S., McGrath, M. J., Ooms, L. M., Gurung, R., Maimone, M. M., Mitchell, C. A. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1: localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication. J. Biol. Chem. 274: 27083-27091, 1999. 2. Emmanuele, V., Kubota, A., Garcia-Diaz, B., Garone, C., Akman, H. O., Sanchez-Gutierrez, D., Escudero, L. M., Kariya, S., Homma, S., Tanji, K., Quinzii, C. M., Hirano, M. Fhl1 W122S causes loss of protein function and late-onset mild myopathy. Hum. Molec. Genet. 24: 714-726, 2015. 3. Fimia, G. M., De Cesare, D., Sassone-Corsi, P. A family of LIM-only transcriptional coactivators: tissue-specific expression and selective activation of CREB and CREM. Molec. Cell. Biol. 20: 8613-8622, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: FHL1

Background:

Synonyms: Four and a half LIM domains protein 1, FHL-1, KyoT, RBP-associated molecule 14-1, RAM14-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1

Tissue Specificity: Isoform 1 seems to be most abundant in each tissue and isoform 2 much less abundant. Isoform 1 is highly expressed in skeletal muscle and lung, and to a lesser extent in heart, brain and kidney. Isoform 2 was found in brain, lung kidney and genital organs.

Background: Four and a half LIM domains protein 1 is a protein that in humans is encoded by the FHL1 gene. This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.

Molecular Weight: 36 kDa

Gene ID: 2273

UniProt: Q13642