IL-10 antibody (AA 137-178)

Catalog No. ABIN7599986

This Rabbit Polyclonal antibody specifically detects IL-10 in IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for IL-10 antibody (AA 137-178) (ABIN7599986)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-IL-10 Antibody

Binding Specificity: AA 137-178

Purpose: Anti-IL-10/IL10 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IL-10/IL10 Antibody Picoband® (ABIN7599986). Tested in ELISA, IHC applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IL-10/IL10 recombinant protein (Position: K137-N178).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL/mL, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Eskdale J, Kube D, Tesch H, Gallagher G (1997). "Mapping of the human IL10 gene and further characterization of the 5' flanking sequence".

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Background:

Synonyms: Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Background: Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Molecular Weight: 19 kDa

Gene ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints