NIP7 antibody (AA 14-180)

Catalog No. ABIN7600009

This anti-NIP7 antibody is a Rabbit Polyclonal antibody detecting NIP7 in WB, ELISA and FACS. Suitable for Human and Mouse.

Quick Overview for NIP7 antibody (AA 14-180) (ABIN7600009)

Target: NIP7 (Nuclear Import 7 Homolog (NIP7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIP7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NIP7 Antibody

Binding Specificity: AA 14-180

Purpose: Anti-NIP7 Antibody Picoband®

Characteristics: Anti-NIP7 Antibody Picoband® (ABIN7600009). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NIP7 recombinant protein (Position: E14-T180). Human NIP7 shares 97.6% and 97% amino acid (aa) sequence identity with mouse and rat NIP7, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 2/23/2021. 2. Liu, J.-F., Wang, X.-Q., Wang, Z.-X., Chen, J.-R., Jiang, T., An, X.-M., Chang, W.-R., Liang, D.-C. Crystal structure of KD93, a novel protein expressed in human hematopoietic stem/progenitor cells. J. Struct. Biol. 148: 370-374, 2004. 3. Morello, L. G., Coltri, P. P., Quaresma, A. J. C., Simabuco, F. M., Silva, T. C. L., Singh, G. Nickerson, J. A., Oliveira, C. C., Moore, M. J., Zanchin, N. I. T. The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing. PLoS One 6: e29174, 2011. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NIP7

Target: NIP7 (Nuclear Import 7 Homolog (NIP7))

Alternative Name: NIP7

Background: 60S ribosome subunit biogenesis protein NIP7 homolog is a protein that in humans is encoded by the NIP7 gene. NIP7 is required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.

Molecular Weight: 20 kDa

Gene ID: 51388

UniProt: Q9Y221

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly