NCOA4 antibody (AA 14-614)

Catalog No. ABIN7600033

This anti-NCOA4 antibody is a Rabbit Polyclonal antibody detecting NCOA4 in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for NCOA4 antibody (AA 14-614) (ABIN7600033)

Target: NCOA4 (Nuclear Receptor Coactivator 4 (NCOA4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NCOA4 Antibody

Binding Specificity: AA 14-614

Purpose: Anti-NCOA4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NCOA4 Antibody Picoband® (ABIN7600033). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NCOA4 recombinant protein (Position: R14-M614).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: NCOA4 Nuclear receptor coactivator 4". 2. Santoro M, Dathan NA, Berlingieri MT, Bongarzone I, Paulin C, Grieco M, Pierotti MA, Vecchio G, Fusco A (Feb 1994). "Molecular characterization of RET/PTC3, a novel rearranged version of the RETproto-oncogene in a human thyroid papillary carcinoma". Oncogene. 9 (2): 509-16. 3. Yeh S, Chang C (May 1996). "Cloning and characterization of a specific coactivator, ARA70, for the androgen receptor in human prostate cells". Proceedings of the National Academy of Sciences of the United States of America. 93 (11): 5517-21.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NCOA4

Target: NCOA4 (Nuclear Receptor Coactivator 4 (NCOA4))

Alternative Name: NCOA4

Background:

Synonyms: Urokinase-type plasminogen activator, U-plasminogen activator, uPA, Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A, Urokinase-type plasminogen activator chain B, PLAU

Tissue Specificity: Expressed in the prostate gland and prostate cancers.

Background: Nuclear receptor coactivator 4 is a protein that in humans is encoded by the NCOA4 gene. This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.

Molecular Weight: 70 kDa

Gene ID: 8031

UniProt: Q13772

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway