RBFA antibody (AA 15-319)

Catalog No. ABIN7600106

Product Details anti-RBFA Antibody

Target: RBFA (Ribosome Binding Factor A (RBFA))

Binding Specificity: AA 15-319

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBFA antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Anti-C18orf22/RBFA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-C18orf22/RBFA Antibody Picoband® (ABIN7600106). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human C18orf22/RBFA recombinant protein (Position: L15-D319).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kuwasako, K. , Suzuki, S. , Nameki, N. , Takizawa, M. , Takahashi, M. , & Tsuda, K. , et al. (2022). 1h, 13c, and 15n resonance assignments and solution structures of the kh domain of human ribosome binding factor a, mtrbfa, involved in mitochondrial ribosome biogenesis. Biomolecular NMR Assignments, 1-7. 2. Rozanska, A. , Richter-Dennerlein, R. , Rorbach, J. , Gao, F. , & Lightowlers, R. N. . (2017). The human rna-binding protein rbfa promotes the maturation of the mitochondrial ribosome. Biochemical Journal, 474(13), 2145-2158. 3. Hauri, S. , Wepf, A. , Drogen, A. V. , Varjosalo, M. , & Gstaiger, M. . (2013). Interaction proteome of human hippo signaling: modular control of the co-activator yap1. Molecular Systems Biology, 9(1), 713.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RBFA

Target: RBFA (Ribosome Binding Factor A (RBFA))

Alternative Name: RBFA (RBFA Products)

Background:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: Predicted to be involved in rRNA processing. Predicted to be located in mitochondrion.

Molecular Weight: 40 kDa

Gene ID: 79863

UniProt: Q8N0V3