PHGDH antibody (AA 15-533)

Catalog No. ABIN7600112

This Rabbit Polyclonal antibody specifically detects PHGDH in WB, ELISA, IHC, FACS, IF, IP and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PHGDH antibody (AA 15-533) (ABIN7600112)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PHGDH Antibody

Binding Specificity: AA 15-533

Purpose: Anti-PHGDH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PHGDH Antibody Picoband® (ABIN7600112). Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PHGDH recombinant protein (Position: L15-F533). Human PHGDH shares 94.4% and 94.2% amino acid (aa) sequence identity with mouse and rat PHGDH, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Acuna-Hidalgo, R., Schanze, D., Kariminejad, A., Nordgren, A., Kariminejad, M. H., Conner, P., Grigelioniene, G., Nilsson, D., Nordenskjold, M., Wedell, A., Freyer, C., Wredenberg, A., and 18 others. Neu-Laxova syndrome is a heterogeneous metabolic disorder caused by defects in enzymes of the L-serine biosynthesis pathway. Am. J. Hum. Genet. 95: 285-293, 2014. 2. Baek, J. Y., Jun, D. Y., Taub, D., Kim, Y. H. Assignment of human 3-phosphoglycerate dehydrogenase (PHGDH) to human chromosome band 1p12 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 89: 6-7, 2000. 3. Benke, P. J., Hidalgo, R. J., Braffman, B. H., Jans, J., van Gassen, K. L. I., Sunbul, R., El-Hattab, A. W. Infantile serine biosynthesis defect due to phosphoglycerate dehydrogenase deficiency: variability in phenotype and treatment response, novel mutations, and diagnostic challenges. J. Child Neurol. 32: 543-549, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH

Background:

Synonyms: PHGDH, PGDH3, D-3-phosphoglycerate dehydrogenase, 3-PGDH, EC 1.1.1.95, 2-oxoglutarate reductase, EC 1.1.1.399, Malate dehydrogenase, EC 1.1.1.37

Background: This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

Molecular Weight: 57 kDa

Gene ID: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect