POT1 antibody (AA 15-638)

Catalog No. ABIN7600116

The Rabbit Polyclonal anti-POT1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect POT1 in samples from Human, Mouse and Rat.

Quick Overview for POT1 antibody (AA 15-638) (ABIN7600116)

Target: POT1 (Protection of Telomeres 1 (POT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-POT1 Antibody

Binding Specificity: AA 15-638

Purpose: Anti-POT1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-POT1 Antibody Picoband® (ABIN7600116). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat POT1 recombinant protein (Position: N15-V638).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL,
1. "Entrez Gene: POT1 POT1 protection of telomeres 1 homolog (S. pombe)". 2. Baumann P, Cech TR (May 2001). "Pot1, the putative telomere end-binding protein in fission yeast and humans". Science 292 (5519): 1171-5. 3. Baumann P, Podell E, Cech TR (Oct 2002). "Human Pot1 (Protection of Telomeres) Protein: Cytolocalization, Gene Structure, and Alternative Splicing". Mol Cell Biol 22 (22): 8079-87.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for POT1

Target: POT1 (Protection of Telomeres 1 (POT1))

Alternative Name: Pot1

Background:

Synonyms: Nucleoprotein TPR, Megator, NPC-associated intranuclear protein, Translocated promoter region protein, TPR

Tissue Specificity: Expressed in esophagus, ovary, liver, skin, smooth muscles, cerebrum and fetal cerebellum (at protein level). Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.

Background: Protection of telomeres protein 1 is a protein that in humans is encoded by the POT1 gene. This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described.

Molecular Weight: 71 kDa

Gene ID: 500054

UniProt: A0A0G231

Pathways: Cell Division Cycle, Telomere Maintenance