CDC7 antibody (AA 152-574)

Catalog No. ABIN7600131

The Rabbit Polyclonal anti-CDC7 antibody has been validated for WB, ELISA and FACS. It is suitable to detect CDC7 in samples from Human.

Quick Overview for CDC7 antibody (AA 152-574) (ABIN7600131)

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-CDC7 Antibody

Binding Specificity: AA 152-574

Purpose: Anti-CDC7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CDC7 Antibody Picoband® (ABIN7600131). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CDC7 recombinant protein (Position: Q152-L574).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hess, G. F., Drong, R. F., Weiland, K. L., Slightom, J. L., Sclafani, R. A., Hollingsworth, R. E. A human homolog of the yeast CDC7 gene is overexpressed in some tumors and transformed cell lines. Gene 211: 133-140, 1998. 2. Jiang, W., Hunter, T. Identification and characterization of a human protein kinase related to budding yeast Cdc7p. Proc. Nat. Acad. Sci. 94: 14320-14325, 1997. 3. Kim, J. M., Sato, N., Yamada, M., Arai, K., Masai, H. Growth regulation of the expression of mouse cDNA and gene encoding a serine/threonine kinase related to Saccharomyces cerevisiae CDC7 essential for G(1)/S transition: structure, chromosomal localization, and expression of mouse gene for S. cerevisiae CDC7-related kinase. J. Biol. Chem. 273: 23248-23257, 1998. Note: Erratum: J. Biol. Chem. 273: 27755 only, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CDC7

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Alternative Name: CDC7

Background:

Synonyms: T-cell surface glycoprotein CD1c, CD1c, CD1C

Tissue Specificity: Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

Background: Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected.

Molecular Weight: 64 kDa

Gene ID: 8317

UniProt: O00311

Pathways: Mitotic G1-G1/S Phases, DNA Replication