BOP1 antibody (AA 157-701)

Catalog No. ABIN7600157

This Rabbit Polyclonal antibody specifically detects BOP1 in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for BOP1 antibody (AA 157-701) (ABIN7600157)

Target: BOP1 (Block of Proliferation 1 (BOP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BOP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-BOP1 Antibody

Binding Specificity: AA 157-701

Purpose: Anti-BOP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-BOP1 Antibody Picoband® (ABIN7600157). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BOP1 recombinant protein (Position: R157-N701). Human BOP1 shares 89.9% and 89.7% amino acid (aa) sequence identity with mouse and rat BOP1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Chen, B., Dragomir, M. P., Fabris, L., Bayraktar, R., Knutsen, E., Liu, X., Tang, C., Li, Y., Shimura, T., Ivkovic, T. C., Cruz de los Santos, M., Anfossi, S., and 36 others. The long noncoding RNA CCAT2 induces chromosomal instability through BOP1-AURKB signaling. Gastroenterology 159: 2146-2195, 2020. 2. Kellner, M., Rohrmoser, M., Forne, I., Voss, K., Burger, K., Muhl, B., Gruber-Eber, A., Kremmer, E., Imhof, A., Eick, D. DEAD-box helicase DDX27 regulates 3-prime end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex. Exp. Cell Res. 334: 146-159, 2015. 3. Killian, A., Le Meur, N., Sesboue, R., Bourguignon, J., Bougeard, G., Gautherot, J., Bastard, C., Frebourg, T., Flaman, J.-M. Inactivation of the RRB1-pescadillo pathway involved in ribosome biogenesis induces chromosomal instability. Oncogene 23: 8597-8602, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for BOP1

Target: BOP1 (Block of Proliferation 1 (BOP1))

Alternative Name: BOP1

Background:

Synonyms: BOP1, KIAA0124, Ribosome biogenesis protein BOP1, Block of proliferation 1 protein

Background: Enables RNA binding activity. Involved in regulation of cell cycle, regulation of signal transduction by p53 class mediator, and ribosomal large subunit biogenesis. Located in chromosome, nucleolus, and nucleoplasm. Part of PeBoW complex.

Molecular Weight: 110 kDa

Gene ID: 23246

UniProt: Q14137