CPN1 antibody (AA 158-208)

Catalog No. ABIN7600158

The Rabbit Polyclonal anti-CPN1 antibody has been validated for WB, ELISA, ICC, FACS and IF. It is suitable to detect CPN1 in samples from Human, Mouse and Rat.

Quick Overview for CPN1 antibody (AA 158-208) (ABIN7600158)

Target: CPN1 (Carboxypeptidase N Subunit 1 (CPN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-CPN1 Antibody

Binding Specificity: AA 158-208

Purpose: Anti-CPN1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CPN1 Antibody Picoband® (ABIN7600158). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CPN1 recombinant protein (Position: D158-N208).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cao, H., Hegele, R. A. DNA polymorphism and mutations in CPN1, including the genomic basis of carboxypeptidase N deficiency. J. Hum. Genet. 48: 20-22, 2003. 2. Gebhard, W., Schube, M., Eulitz, M. cDNA cloning and complete primary structure of the small, active subunit of human carboxypeptidase N (kininase 1). Europ. J. Biochem. 178: 603-607, 1989. 3. Hegele, R. A. Personal Communication. London, Ontario, Canada 2/24/2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CPN1

Target: CPN1 (Carboxypeptidase N Subunit 1 (CPN1))

Alternative Name: CPN1

Background:

Synonyms: N-alpha-acetyltransferase 15, NatA auxiliary subunit,Gastric cancer antigen Ga19,N-terminal acetyltransferase,NMDA receptor-regulated protein 1,Protein tubedown-1,Tbdn100,NAA15,GA19, NARG1, NATH, TBDN100,

Tissue Specificity: Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. .

Background: Carboxypeptidase N catalytic chain is an enzyme that in humans is encoded by the CPN1 gene. Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits, this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency.

Molecular Weight: 52 kDa

Gene ID: 1369

UniProt: P15169

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process